Effect of Superoxide Generation and Dismutation on Hydroxylation Reactions Catalyzed by Liver Microsomal Cytochrome P-450
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چکیده
منابع مشابه
Hydrogen peroxide formation and stoichiometry of hydroxylation reactions catalyzed by highly purified liver microsomal cytochrome P-450.
The stoichiometry of hydroxylation reactions catalyzed by cytochrome P-450 was studied in a reconstituted enzyme system containing the highly purified cytochrome from phenobarbital-induced rabbit liver microsomes. Hydrogen peroxide was shown to be formed in the reconstituted system in the presence of NADPH and oxygen; the amount of peroxide produced varied with the substrated added. NADPH oxida...
متن کاملStudies on hydroperoxide-dependent substrate hydroxylation by purified liver microsomal cytochrome P-450.
Highly purified liver microsomal cytochrome P-450 catalyzes the hydroperoxide-dependent hydroxylation of a variety of substrates in the absence of NADPH, NADPHcytochrome P-450 reductase, and molecular oxygen. The addition of phosphatidylcholine is necessary for maximal activity. The absence of flavoproteins and cytochrome b, from the cytochromeP-450 preparations rules out the involvement of oth...
متن کاملOn the stoichiometry of the oxidase and monooxygenase reactions catalyzed by liver microsomal cytochrome P-450. Products of oxygen reduction.
This laboratory has recently reported that, in a reconstituted enzyme system containing alcohol-induced isozyme 3a of liver microsomal cytochrome P-450, the sum of acetaldehyde generated by the monooxygenation of ethanol and of hydrogen peroxide produced by the NADPH oxidase activity is inadequate to account for the O2 and NADPH consumed. Studies on the stoichiometry have revealed the occurrenc...
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sideration in the literature [13]. As a result, the findings obtained in this investigation may be applicable to the study of PMA in other cell systems. According to Augustinsson [S], each esterase type exists in multiple forms and each animal species has its own typical spectrum of esterases in plasma and tissues [14]. In the presence of marked variations in location, form and activity, one mi...
متن کاملAliphatic hydroxylation by highly purified liver microsomal cytochrome P-450. Evidence for a carbon radical intermediate.
The oxidation of norbornane by a reconstituted liver cytochrome P-450 system affords exoand endo-2-norborneol in a ratio of 3.4:1. The ratio ofese products was found to be 0.76:l when exo!exo,exo,exo-2,3,5,6-tetradueteronorbornane --was oxidized. Analysis of the mass spectra of the products from the deuterated hydrocarbon showed that 25% of the exo-norborneol contained four deuterium atoms wher...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)45853-5